Urine DNA 96-Well Isolation Kit (Slurry Format), 1 plate, Manufacturer reference: 27100

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified with a rapid and convenient spin column protocol. Multiple samples can be processed in 45 minutes. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications such as PCR, qPCR, DNA fingerprinting, methylation studies and more. This kit can also be used to isolate DNA from a broad range of viruses. 

This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.

Background

Many advantages favour the use of urinary DNA for cancer biomarker discovery and diagnosis of different pathogens over the use of blood and tissue samples. DNA found in urine can be divided into 2 basic categories. The larger genomic DNA (gDNA) species is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid. Both types of DNA can be isolated reliably using this kit.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25oC) for up to 1 year without showing any reduction in performance. It is recommended to warm up Solution A and Solution B for 20 minutes at 60oC if any salt precipitation is observed.