HOT FIREPol Probe qPCR Mix

5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

 

For in vitro use only

 

Description:

HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. 


HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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