FIREScript RT cDNA synthesis Kit
The kits contain:
1 tube FIREScript RT enzyme 200U/µl;
1 tube 10x RT Reaction Buffer with MgCl2 and DTT;
1 tube 20mM dNTPs;
1 tube 100µM oligo (dT);
1 tube 100µM Random Primers;
H2O (1-3 tubes)
User needs to add:
•Template RNA
•Specific primer (ONLY if Oligo(dT) or Random is not used)
•RiboGrip, Ribonuclease Inhibitor
