Routine PCR & Master Mixes
For in vitro use only
FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol®has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.
Exceptional stability at ambient temperature
Selection of buffers and an enhancer for
Extension rate 2-4 kb/min at 72°C
Error rate per nucleotide per cycle: ~2.5 x 10-5
Estimated half life at 95°C is 1.5 hours
Wide range of PCR assays
Amplification efficiency ≥ 105 fold
Functional quality control via PCR on different templates
No ds Endodeoxyribonuclease activity
No ss Exodeoxyribonuclease activity
No self-priming activity
We supply FIREPol® Polymerase as enzyme kit with a selection of buffers and an enhancer for personal optimization.
Master Mixes with or without ready-to-load feature are also available.
FIREPol® DNA Polymerase
5x FIREPol® Master Mix (7.5 mM MgCl2)
5x FIREPol® Master Mix (12.5 mM MgCl2)
5x FIREPol® Master Mix Ready to Load (7.5 mM MgCl2)
5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2)