Routine PCR Enzymes & Master Mixes

Routine PCR & Master Mixes

 

FIREPol® DNA Polymerase

 For in vitro use only 

 

Description: 

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol®has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

 

Features

  • Exceptional stability at ambient temperature

  • Selection of buffers and an enhancer for

    personal optimization

  • Extension rate 2-4 kb/min at 72°C

  • Error rate per nucleotide per cycle: ~2.5 x 10-5

  • Estimated half life at 95°C is 1.5 hours

Applications
  • Wide range of PCR assays

  • TA cloning

     

 
Control
  • Amplification efficiency ≥ 105 fold

  • Functional quality control via PCR on different templates

  • No ds Endodeoxyribonuclease activity

  • No ss Exodeoxyribonuclease activity

  • No self-priming activity 

 

We supply FIREPol® Polymerase as enzyme kit with a selection of buffers and an enhancer for personal optimization.

Master Mixes with or without ready-to-load feature are also available.

 

ProductData sheetMSDSCoA

 FIREPol® DNA Polymerase

       PDF    PDF   PDF

 5x FIREPol® Master Mix (7.5 mM MgCl2)

        PDF    PDF   PDF

 5x FIREPol® Master Mix (12.5 mM MgCl2)

        PDF    PDF   PDF

 5x FIREPol® Master Mix Ready to Load (7.5 mM MgCl2)

        PDF    PDF   PDF

 5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2)

        PDF    PDF   PDF
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