cDNA Synthesis
cDNA synthesis (reverse transcription)
comparison of FIREScript and SOLIScript:
| FIREScript RT | SOLIScript RT | |
|
Excellent enzyme for standard applications |
Superior specificity with gene specific primers thanks to higher working temperature |
|
|
Origin |
MMLV-based |
In silico designed |
|
Reaction temperature |
Up to 50C |
Up to 60C |
|
Suitable primers |
Random Oligo dT Gene specific |
Oligo dT Gene specific |
|
RNase H activity |
Full |
Reduced |
|
Full length cDNA |
Yes |
Yes |
|
Reaction set-up at room temperature |
Yes |
Yes |
