cDNA Synthesis

cDNA synthesis (reverse transcription)

comparison of FIREScript and SOLIScript:

FIREScript RT SOLIScript RT

Excellent enzyme for standard applications

Superior specificity with gene specific primers thanks to higher working temperature

Origin

MMLV-based

In silico designed

Reaction temperature

Up to 50C

Up to 60C

Suitable primers

Random

Oligo dT

Gene specific

Oligo dT

Gene specific

RNase H activity

Full

Reduced

Full length cDNA

Yes

Yes

Reaction set-up at room temperature

Yes

Yes

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