08-61-00250, SOLIScript® 1-step Multiplex Probe Kit (Purple), 250rxn
SOLIScript 1-step Multiplex Probe Kit (Purple)
For in vitro use only
Description:
SOLIScript 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.
Applications:
- Detection and quantification of RNA targets with qPCR
- DNA/LNA hydrolysis probe based assays
- Suitable for singleplex and multiplex reactions
Reagents Provided:
- 5x One-step Multiplex Probe Mix (Purple)
HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), Purple dye
If Cy5 dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available.
- 40x One-step SOLIScript Mix
SOLIScript Reverse Transcriptase, RiboGrip RNase Inhibitor
- Water, nuclease free
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
| Manufacturer | Solis Biodyne |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |