16907-01,     IMMERSION OIL TYPE A

16907-01, IMMERSION OIL TYPE A

16908,     IMMERSION OIL TYPE B, 4 oz

16908, IMMERSION OIL TYPE B, 4 oz

16907-16, IMMERSION OIL TYPE A

For Normal Light Microscopy

Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.

€62.98
SKU
EMS_16907-16

Immersion Oils For Normal Light Microscopy

Which Type - Which Application?

Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria. Now four standard types are available with the introduction of Type NVH, a very high viscosity oil. All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.)

arrow12For Normal Light Microscopy

Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.

arrow1237°C Immersion Oils

A number of research projects require microscopic studies at internal body temperature. Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates. This problem has been resolved by the formation of Cargille 37°C Immersion Oils, which have an index of 1.515 at 37°C.

Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.

arrow12Immersion Oil Type 300

Automated Hematology Systems: Use Type 300; Automated Hematology Systems depend on accurate, precisely controlled physical and optical properties of immersion oil for successful imaging and mechanical processing. Type 300 is designed and manufactured to meet the stringent requirements of this equipment, which include specialized viscosity and exacting controls for its consistency.

Immersion Oils Specifications

OPTICAL & PHYSICAL PROPERTIES
Immersion Oil Type A B 300
Refractive Index : @23°C @23°C @23°C
  F Line (4861 A) 1.5239 1.5236 1.5238
  E Line (5461 A) 1.5180 1.5180 1.5180
  D Line (5893 A) 1.5150 1.5150 1.5150
  C Line (6563 A) 1.5114 1.5115 1.5116
Dispersion:      
  nF-nC 0.0125 0.0119 0.0123
  Abbe VD 41.3 43.2 41.8
  Abbe Ve 41.0 42.8 41.5
Temperature Coefficient (15-35°C) -0.00033 -0.00031 -0.00033
Stability (change in nD25°C after 24 hrs @ temp.)      
  at 60°C: 0 0 0
  at 100°C: 0 0 0
Fluorescence (1): Short UV: low low low
Fluorescence (1): Long UV: low low low
Color: (Gardner) 1 1 1
Viscosity:  cSt +/- 10% @ 23°C (centistokes) 150
low
1250
high
300
medium
Density: @ 23°C g/cc: .923 .923 .923
Density: (US) lb/gal 7.70 7.70 7.70
Cloud Point: <-13°C <-13°C <-13°C
Flash Point: 325°F 325°F 325°F
Neutralization No.: (mg KOH/g) 0.01 max 0.01 max 0.01 max
More Information
Manufacturer Electron Microscopy Sciences
Size / Rxns Number: No
Protocol & Manual No
Technical Tips No
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