This diamond knife has a diamond platform which ensures easy and gentle sectioning for cryo-immunocytochemistry.
Sections are collected directly from the diamond surface using a loop (AGT5112) and a droplet of sucrose/methyl cellulose. The 35° cutting angle leads to a reduction in mechanical stresses and thus improved structural preservation in sucrose protected samples.
Available in 2.0 and 3.0mm sizes.
Cutting range: 30 nm - 1 µm
cryo immunoThe first cryo knife with a diamond platform, guarantees the best possible sectioning for sucrose infiltrated samples (Tokuyasu).The diamond platform guarantees an easy and gentle section pick-up. The sections are collected directly from the diamond surface using a loop and a sucrose/methyl-cellulose droplet (W. Liou et al., Histochemistry and Cell Biology, Vol. 106, pp. 41 – 55, 1996. P. J. Peters et al., Current Protocols in Cell Biology, pp. 4.7.1 – 4.7.18, 2006). The 35° angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose infiltrated samples (E. Bos et al., Journal of Structural Biology 175, pp. 62 – 72, 2011). We recommend the cryo immuno knife also for sectioning frozen hydrated samples (CEMOVIS). The 35° angle is a good compromise between durability and cutting performance (A. Al-Amoudi et al., Journal of Structural Biology 150, pp. 109 – 121, 2005). |
 
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| Manufacturer | DiATOME Ltd |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | https://www.diatome.ch/Messerprospekt_01_2015_EN_LowRes.pdf |
| Technical Tips | No |
