Southern Blot Analysis, Manufacturer reference: 207
ABOUT THIS PRODUCT:
This experiment introduces your students to Southern blotting as a tool for "DNA Fingerprinting" in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.
DOWNLOAD SAMPLE INSTRUCTIONS
DOWNLOAD SAMPLE INSTRUCTIONS
€170.00
SKU
EDV_207
Details
Group Size:
For 5 lab groups
Time Required:
Electrophoresis - 45 minutes / Blot - overnight / Stain/Destain - 10 minutes
Kit Includes:
Instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™.
All You Need:
Electrophoresis apparatus, power supply, 65º C Water bath, DNA visualization system, staining net & tray, automatic micropipettes, lab glassware, microwave or hot plate, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, forceps
Replenisher:
- #207 FB - EDVO-Kit #207 Replenisher (Comp. A-E)
Resources
| Manufacturer | Edvotek Inc. |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |
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