Transformation of E. coli with pGAL™, Manufacturer reference: 221
ABOUT THIS PRODUCT:
In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.
No IPTG used in this experiment.
DOWNLOAD SAMPLE INSTRUCTIONS
No IPTG used in this experiment.
DOWNLOAD SAMPLE INSTRUCTIONS
€170.00
SKU
EDV_221
Details
Group Size:
For 10 lab groups
Time Required:
Complete in 50 minutes and grow overnight
Kit Includes:
Instructions, BactoBeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, petri dishes, sterile pipettes, loops and microtubes.
All You Need:
Automatic micropipette (5-50µl) and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves
Storage:
Some Components Require Refrigerator and Freezer Storage
Replenisher:
- #221 FB - EDVO-Kit #221 Replenisher (Comp. B-E)
- #726 - BactoBeads™ E. coli JM109
Resource
Quick Guides:
Troubleshooting Guides:
Safety Data Sheets:
Miscellaneous:
Instructional Videos:
| Manufacturer | Edvotek Inc. |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |
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