Transformation of E. coli with Green Fluorescent Protein, Manufacturer reference: 223

Details

Group Size:

For 10 lab groups

Time Required:

Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutes

Kit Includes:

Instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes

All You Need:

Automatic micropipette (5-50 µl) and tips, two water baths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light

Storage:

Some Components Require Refrigerator and Freezer Storage

Replenisher:

• #223 FB - EDVO-Kit #223/AP08 Replenisher (Comp. B-D + Growth Additive)
• #728 - BactoBeads™ E. coli GFP Host

Resource

Quick Guides:

• Transformation

Troubleshooting Guides:

• Transformation Troubleshooting

Safety Data Sheets:

• EDVO-Kit #223/AP08 SDS

Miscellaneous:

• pFluoroGreen Plasmid Map

Instructional Videos:

• Genetic Engineering & Transformation