Fingerprinting of Bacterial Proteins, Manufacturer reference: 252

Details

Group Size:

For 6 groups sharing 3 gels

Time Required:

Day One Set Up - 15 minutes / Day Two Set Up - 60 minutes / Electrophoresis - 60 minutes / Staining - 20 minutes / Destaining - 2 hours

Kit Includes:

Instructions, bacterial cultures and reagents, Lysozyme, buffers, Protein InstaStain®, practice gel loading solution, protein sample buffer, unknown proteins ready for electrophoresis, ReadyPour™ Agar, nutrient broth, screw cap tubes, sterile inoculating loops, sterile 1 ml pipets, sterile petri plates, 100 x 15 mm

All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, microcentrifuge, incubation oven, hot plate or burner, white light visualization system, automatic micropipette with fine tips, lab glassware, methanol, glacial acetic acid, distilled water, sterile water or nutrient broth, plastic wrap, photodocumentation system (optional)

Storage:

Some Components Require Freezer Storage

Replenisher:

• #252 FB - EDVO-Kit #252 Replenisher (Comp. D-G)
• #252 BactoBeads™ - EDVO-Kit #252 Replenisher (BactoBeads™)

Resource

Quick Guides:

• Protein Guide

Troubleshooting Guide:

• Protein Troubleshooting

Safety Data Sheets:

• EDVO-Kit #252 SDS

Instructional Videos:

• SDS PAGE