Purification & Size Determination of Green & Blue Fluorescent Proteins, Manufacturer reference: 255
ABOUT THIS PRODUCT:
When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP will be used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP will be identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
DOWNLOAD SAMPLE INSTRUCTIONS
DOWNLOAD SAMPLE INSTRUCTIONS
€136.00
SKU
EDV_255
Details
Group Size:
For 6 lab groups
Time Required:
Packing & Running Column - 45 minutes / Optional Electrophoresis - 60 minutes / Staining - 30 minutes / Destaining - 2 hours
Kit Includes:
Instructions, columns and matrix, GFP and BFP extracts, buffer, protein gel reagents for optional activity.
All You Need:
Water bath, long wave UV lamp, ring stand; clamps, automatic micropipette, vertical gel electrophoresis apparatus, power supply, hot plate, polyacrylamide gels (12%).
Storage:
Some Components Require Freezer Storage
Replenisher:
Resource
Safety Data Sheets:
Instructional Videos:
| Manufacturer | Edvotek Inc. |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |
Write Your Own Review