Blue/White Cloning of a DNA Fragment and Assay of B-galactosidase, Manufacturer reference: 300

Details

PRODUCT UPDATE:

Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.

Group Size:

For 5 lab groups

Time Required:

Complete three modules in 3 hours 10 minutes

Kit includes:

Instructions, Linearized pUC plasmid & DNA fragment, T4 Ligase, BactoBeads™ for transformation, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies

You Need:

Incubation oven, two water baths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice.

Storage:

Some Components Require Refrigerator and Freezer Storage

Replenisher:

• #300 FB - EDVO-Kit #300 Replenisher (Comp. L1-L3, TR1-TR5, A2, A4)
• #726 - BactoBeads™ E. coli JM109

Resource

Safety Data Sheets:

• EDVO-Kit #300 SDS

Instructional Videos:

• Genetic Engineering & Transformation