The Mother of All Experiments: Exploring Human Origin by PCR Amplification of Mitochondrial DNA, Manufacturer reference: 332

Details

Group Size:

For 25 students

Time Required:

Extraction - 50 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 minutes to overnight

Kit Includes:

Instructions, PCR EdvoBeads™ PLUS, Universal DNA Buffer, TE Buffer, Mitochondrial LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Replenisher:

• #332 FB - EDVO-Kit #332 Replenisher (Comp. A-E)
• #PCR EdvoBeads™ PLUS - PCR EdvoBeads™ PLUS Replenisher

Resource

Quick Guides:

• Polymerase Chain Reaction

Troubleshooting Guides:

• PCR & Electrophoresis Troubleshooting
• DNA Extraction & PCR Troubleshooting

Safety Data Sheets:

• EDVO-Kit #332 SDS

Instructional Videos:

• Polymerase Chain Reaction
• Electrophoresis
• Visualizing DNA