Lighting Up life: Expression of GFP in c. elegans, Manufacturer reference: 858

Group Size:

For 10 lab groups

Time Required:

Growing Bacteria - Overnight
Plating Worms - 15 minutes
Worm Growth - 3-4 Days
Set Up - 30 minutes
PCR - 2 hours or overnight
Electrophoresis - 90 minutes

Kit Includes:

Instructions, C. elegans, E. coli OP50 cells, PCR EdvoBeads™ PLUS, GFP primer mix concentrate, EdvoQuick™ DNA ladder, control DNA concentrate, rehydration buffer, sterile water, TE buffer, extraction buffer, ReadyPour™ NGM agar, NGM salts, petri dishes, pipets, tubes, UltraSpec-Agarose™, electrophoresis buffer (50x), 10x gel loading solution, SYBR® Safe DNA stain, FlashBlue™ liquid stain, sterile loops, & wax beads

All You Need:

Covered box, fluorescent and dissecting microscopes, timers, water bath, incubator (optional), thermal cycler or three water baths, electrophoresis apparatus, power supply, balance, microcentrifuge, UV transilluminator or UV photodocumentation system, automatic micropipettes with tips, microwave or hot plate, pipet pump, permanent markers, flasks or beakers, hot gloves, distilled or deionized water, sterile water, ice buckets and ice, bleach solution or laboratory disinfectant

Storage:

Some Components Require Refrigerator & Freezer Storage

Replenisher:

• #858 FB - EDVO-Kit #858 Replenisher (Comp. B-D + TE Buffer)
• #858 BC - NGM Medium Components
• #PCR EdvoBeads™ PLUS - PCR EdvoBeads™ PLUS Replenisher

Resource

Quick Guides:

• Polymerase Chain Reaction

Troubleshooting Guides:

• PCR & Electrophoresis Troubleshooting
• Care and Feeding of C. elegans

Safety Data Sheets:

• EDVO-Kit #858 SDS

Instructional Videos:

• Model Organisms
• Polymerase Chain Reaction
• Electrophoresis
• Visualizing DNA