Biotechnology - Restriction Enzyme Analysis of DNA, Manufacturer reference: AP09
ABOUT THIS PRODUCT:
Analysis of Eco RI Cleavage Patterns of Lambda DNA
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
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This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
DOWNLOAD SAMPLE INSTRUCTIONS
€128.00
SKU
EDV_AP09
Details
Group Size:
Time Required:
Kit Includes:
All You Need:
Storage:
For 8 Gels
Time Required:
Complete in 60 minutes
Kit Includes:
Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 ml (or 0.5 ml) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain® Blue Cards, & Disposable Pipets.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Storage:
Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
Quick Guides:
- Agarose Gel Electrophoresis
- Visualizing DNA
- Micropipetting Basics
- Using Technology to Create a DNA Standard Curve
Safety Data Sheets:
Instructional Videos:
| Manufacturer | Edvotek Inc. |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |
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