After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.
Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.
For Horizontal, Inverted and Inclined Instruments and Projection Equipment
Higher viscosities are generally required. Type NVH meets the requirement, having a viscosity of 21,000cST.
| Immersion Oil Type | 37** | NVH | OVH | |
| Refractive Index : | @37°C | @23°C | @23°C | |
| F Line (4861 A) | 1.5238 | 1.5230 | 1.5230 | |
| E Line (5461 A) | 1.5180 | 1.5178 | 1.5178 | |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 | |
| C Line (6563 A) | 1.5115 | 1.5118 | 1.5118 | |
| Dispersion: | ||||
| nF-nC | 0.0123 | 0.0113 | 0.0111 | |
| Abbe VD | 42.0 | 45.7 | 46.3 | |
| Abbe Ve | 41.7 | 45.4 | 46.0 | |
| Temperature Coefficient (15-35°C) | -0.00035 | -0.00034 | -0.00034 | |
| Stability (change in nD25°C after 24 hrs @ temp.) | ||||
| at 60°C: | 0 | 0 | 0 | |
| at 100°C: | 0 | 0 | 0 | |
| Fluorescence (1): Short UV: | low | low | low | |
| Fluorescence (1): Long UV: | low | low | low | |
| Color: (Gardner) | 1 | 1 | <3 | |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 1250 high |
21,000 very high |
46,000 very high |
|
| Density: @ 23°C g/cc: | 0.917 | 0.919 | 0.918 | |
| Density: (US) lb/gal | 7.73 | 7.67 | 7.66 | |
| Cloud Point: | <-13°C | <-13°C | <-13°C | |
| Flash Point: | 325°F | 325°F | 325°F | |
| Neutralization No.: (mg KOH/gm) | 0.01 max | 0.01 max | 0.04 max |
|
| Manufacturer | Electron Microscopy Sciences |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |