Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.
After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.
Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.
Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.
Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.
| Immersion Oil | Type LDF | Type FF | Type HF | ||
| Refractive Index @ 23°C: | |||||
| F Line (4861 A) | 1.5239 | 1.4850 | 1.5234 | ||
| E Line (5461 A) | 1.5181 | 1.4811 | 1.5180 | ||
| D Line (5893 A) | 1.5150 | 1.4790 | 1.5150 | ||
| C Line (6563 A) | 1.5115 | 1.4766 | 1.5117 | ||
| Dispersion: | |||||
| nF-nC | 0.0125 | 0.0084 | 0.0118 | ||
| Abbe VD | 41.3 | 57.0 | 43.8 | ||
| Abbe Ve | 41.0 | 56.5 | 43.6 | ||
| Temperature Coefficient (15-35°C) |
-0.00036 | -0.00037 | -0.00038 | ||
| Stability (change in nD25°C after 24 hrs. @ temp.) | |||||
| at 60°C: | -0.0004 | 0 | +0.0001 | ||
| at 100°C: | -0.0043 | 0 | +0.0006 | ||
| Fluorescence (1): | |||||
| Short UV: | very low | none | verylow | ||
| Long UV: | very low | none | verylow | ||
| Color: (Gardner) | <1 | <1 | <1 | ||
| Viscosity: +/-10% @ 23°C (centistokes) | 500 medium |
170 low |
700 medium |
||
| Density: | |||||
| @ 23°C g/cc: | 0.984 | 0.877 | .9306 | ||
| (US) lb/gal | 8.21 | 7.32 | 7.77 | ||
| Cloud Point: | <-14°C | <-6°C | <2°C | ||
| Flash Point: | 171°F | 420°F | >340°F | ||
| Neutralization No.: (mg KOH/gm) | <0.01 max | 0.01max | 0.03max | ||
| Manufacturer | Electron Microscopy Sciences |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |