Methylene Blue Chloride, Certified, C.N. #DcA-24 (Basic Blue 9; Swiss Blue) C16H18N3SCl •3H2O F.W. 319.86+aq CAS #7220-79-3 Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve™; 10.0% Glycol; 0.0% Xylene. Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231. General stain for animals, histology. A simple rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and Methylene Blue. Dougherty, M.M., and King, J.S., (1984) Stain Tech., 59, 149 An established routine method for differential staining of epoxy-embedded tissue sections. Schroeder, H.E., Rossinsky, K., and Muller, W., (1980) Microsc. Acta 83, 111 Used with Basic Fuchsin for stain epoxy-embedded tissue. Sato, T. and Shamoto, M. (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol., 48:223