Group Size:
For 5 lab groups
Time Required:
Complete in 5-6 hours
Kit Includes:
Instructions, BactoBeads™ with purple and pink plasmid, LB Growth Media, Ampicillin, IPTG, protein extraction buffer, wash buffer, elution buffer, dry ion exchange matrix, standard protein markers, 50% glycerol solution, protein denaturing solution, microcentrifuge Tubes, pH paper, centrifuge tubes, chromatography columns, protein Protein InstaStain®, practice gel loading solution, tris-glycine-SDS electrophoresis buffer, transfer pipets, loops
All You Need:
Variable micropipettes, centrifuge, vertical gel electrophoresis apparatus and power supply, waste container, ring stands and column clamps, stir plate and stir bars, thermometers, graduated cylinders, Erlenmeyer flasks, 70% ethanol, distilled water, spectrophotometer and cuvettes, aluminum foil, vortex, lab markers, water bath, 3 polyacrylamide gels, glacial acetic acid, methanol, air pump (optional), shaker incubator (optional), autoclave or oven (optional)
Storage:
Some Components Require Refrigerator and Freezer Storage
Replenisher:
| Manufacturer | Edvotek Inc. |
|---|---|
| Size / Rxns Number: | No |
| Protocol & Manual | No |
| Technical Tips | No |