Reverse Transcription PCR (RT-PCR): The Molecular Biology of HIV Replication, Manufacturer reference: 335*

Details

Group Size:

For 6 lab groups

Time Required:

PCR Setup - 10 minutes
PCR - 3 hours
Electrophoresis - 60 minutes
Staining - 5 minutes

Kit Includes:

Instructions, RNA LyphoTemplate™, LyphoPrimer™ mix, tubes with RT-PCR EdvoBeads™, RNAse-free water, EdvoQuick™ DNA Ladder, UltraSpec-Agarose™, 50X Electrophoresis Buffer, 10X Gel Loading Solution, SYBR® Safe stain, microcentrifuge & PCR tubes.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (3) Water Baths (if performing experiment without a thermal cycler), Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Replenisher:

• #335 FB - EDVO-Kit #335 Replenisher (Comp. A-D)
• #335 PCR - EDVO-Kit #335 Replenisher (RT-PCR Bag)

Resources

Quick Guides:

• Polymerase Chain Reaction

Troubleshooting Guides:

• PCR & Electrophoresis Troubleshooting

Safety Data Sheets:

• EDVO-Kit #335 SDS

Instructional Videos:

• Polymerase Chain Reaction
• Electrophoresis
• Visualizing DNA