Drosophila Genotyping Using PCR, Manufacturer reference: 337*

Details

Group Size:

For 10 lab groups

Time Required:

Extraction - 50 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 minutes to overnight

Kit Includes:

Instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, EdvoQuick™ DNA ladder, Drosophila LyphoPrimer™, Drosophila WT LyphoTemplate™, potassium acetate, DNA extraction buffer, Proteinase K, Wild-type and White Drosophila, UltraSpec-Agarose™, electrophoresis buffer (50x), SYBR® Safe stain, FlashBlue™ liquid stain, microcentrifuge & PCR tubes.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), Water Bath, Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Replenisher:

• #337 FB - EDVO-Kit #337 Replenisher (Comp. A-G + PROK)
• #PCR 25 - EDVO-Kit PCR Bag Replenisher

Resources

Quick Guides:

• Polymerase Chain Reaction

Troubleshooting Guides:

• PCR & Electrophoresis Troubleshooting

Safety Data Sheets:

• EDVO-Kit #337 SDS

Instructional Videos:

• Polymerase Chain Reaction
• Electrophoresis
• Visualizing DNA